Methods for in vitro CRISPR/CasRx-Mediated RNA Editing
نویسندگان
چکیده
Specific changes in the genome have been accomplished by revolutionary gene-editing tool known as clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system. The advent of programmable RNA editing CRISPR/Cas nucleases has made this safer and more precise. Specifically, CasRx, a family member Cas13d family, shown great therapeutic potential. Here, we describe vitro methods utilizing powerful platform determine efficiencies for CasRx with different forms guide RNAs (also gRNA or sgRNA).
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ژورنال
عنوان ژورنال: Frontiers in Cell and Developmental Biology
سال: 2021
ISSN: ['2296-634X']
DOI: https://doi.org/10.3389/fcell.2021.667879