Methicillin Resistant Staphylococcus aureus (MRSA) Isolation and mecA Gene Detection from Milk and Farmer Hand Swab in Tulungagung, Indonesia

Staphylococcus aureus is a harmful bacterium that often contaminates milk; hence it believed to become severe health risk for humans . S. resistant β‑lactam drugs can be termed methicillin (MRSA). Dairy farms have high incidence of MRSA infections due the repeated use same medicines on dairy cows and physical contact between farmers during milking. This study looked in cow milk farmer hand swabs Tulungagung, Indonesia. Using oxacillin cefoxitin diffusion disks, phenotypic detection approaches were evaluated, then transferred Oxacillin Resistance Screening Agar Base (ORSAB) test genotypically verified using PCR find mec A gene encoding MRSA. One hundred ten samples 45 farmer's collected from East Java, Mannitol salt agar (MSA) was used cultivation purification. The disk-diffusion identify resistance. cefoxitin-resistant isolates tested ORSAB. In addition, PCR-tested gene. found 110 (70.97%) 155 isolates. Of total , 16 (14.54%) 39 (35.45%) known Cefoxitin Oxacillin, respectively. When with ORSAB, 23 55 showed positive results source most which 15 isolates, while only carried 8 However, analysis two According this study, many Indonesia, but few mecA