Metasurface integrated with double-helix point spread function and metalens for three-dimensional imaging
نویسندگان
چکیده
منابع مشابه
Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function.
We demonstrate single-molecule fluorescence imaging beyond the optical diffraction limit in 3 dimensions with a wide-field microscope that exhibits a double-helix point spread function (DH-PSF). The DH-PSF design features high and uniform Fisher information and has 2 dominant lobes in the image plane whose angular orientation rotates with the axial (z) position of the emitter. Single fluorescen...
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Optical imaging of single biomolecules and complexes in living cells provides a useful window into cellular processes. However, the three-dimensional dynamics of most important biomolecules in living cells remains essentially uncharacterized. The precise subcellular localization of mRNA-protein complexes plays a critical role in the spatial and temporal control of gene expression, and a full un...
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Single-particle tracking has been applied to study chromatin motion in live cells, revealing a wealth of dynamical behavior of the genomic material once believed to be relatively static throughout most of the cell cycle. Here we used the dual-color three-dimensional (3D) double-helix point spread function microscope to study the correlations of movement between two fluorescently labeled gene lo...
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Three-dimensional nanoscale localization and tracking of dim single emitters can be obtained with a widefield fluorescence microscope exhibiting a double-helix point spread function (DH-PSF). We describe in detail how the localization precision quantitatively depends upon the number of photons detected and the z position of the nanoscale emitter, thereby showing a approximately 10 nm localizati...
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We present a new method with which to binarize pupil filters designed to control the three-dimensional irradiance distribution in the focal volume of an optical system. The method is based on a one-dimensional iterative algorithm, which results in efficient use of computation time and in simple, easy to fabricate binary filters. An acceptable degree of resemblance between the point-spread funct...
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ژورنال
عنوان ژورنال: Nanophotonics
سال: 2019
ISSN: 2192-8614
DOI: 10.1515/nanoph-2018-0216