Metallothionein Gene from Tetrahymena thermophila with a Copper-Inducible-Repressible Promoter
نویسندگان
چکیده
منابع مشابه
A robust inducible-repressible promoter greatly facilitates gene knockouts, conditional expression, and overexpression of homologous and heterologous genes in Tetrahymena thermophila.
The Cd(2+)-inducible metallothionein (MTT1) gene was cloned from Tetrahymena thermophila. Northern blot analysis showed that MTT1 mRNA is not detectable in the absence of Cd(2+), is induced within 10 min of its addition, is expressed in proportion to its concentration, and rapidly disappears upon its withdrawal. Similarly, when the neo1 gene coding region flanked by the MTT1 gene noncoding sequ...
متن کاملMultiple introns in a conjugation-specific gene from Tetrahymena thermophila.
Multiple introns have been found in a gene from a ciliated protozoan. This Tetrahymena thermophila gene (cnjB) is large (7.5 kb mRNA) and active only during conjugation, the organism's sexual cycle. Six introns ranging in size from 62 bp to 676 bp were found when we sequenced a 3.1 kb segment of the cnjB gene together with its corresponding cDNA. We estimate, by extrapolation of our current dat...
متن کاملCytogamy: An Inducible, Alternate Pathway of Conjugation in TETRAHYMENA THERMOPHILA.
We report the occurrence of cytogamy in Tetrahymena thermophila. By analogy to Paramecium, cytogamy generates exconjugant clones that derive their entire genetic information from a single meiotic product of their cytoplasmic parent. Thus, "instant" whole-genome homozygotes are created. Cytogamy has been induced in every strain of T. thermophila tested, and most of the excytogamous progeny have ...
متن کاملTetrahymena thermophila
What does it look like? Tetrahymena thermophila is a unicellular eukaryote which is larger than many mammalian cells (~30 × 50 μm). It swims in temperate freshwater environments, waving its coat of cilia (Figure 1, inset) and phagocytosing food propelled into its gullet. Hunger induces Tetrahymena cells to undergo a transformation, including growth of a long posterior cilium and more coordinate...
متن کاملAdolescence in Tetrahymena thermophila.
The development of sexual maturity in Tetrahymena thermophila has been shown to include an intermediate stage, adolescence, during which cells are capable of mating with mature cells but not other adolescent cells. When the progeny of successfully mated cells are grown logarithmically and tested frequently for the ability to mate, they are unable to form mating pairs for about 65 generations. T...
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ژورنال
عنوان ژورنال: Eukaryotic Cell
سال: 2006
ISSN: 1535-9778,1535-9786
DOI: 10.1128/ec.5.2.422-425.2006