Metagenomics of mine tailing rhizospheric communities and its selection for plant establishment towards bioremediation

Mining operations often generate tailing dams that contain toxic residues and are a source of contamination when left unconfined. The establishment plant community over the tailings has been proposed as containment strategy known phytostabilization. Previously, we described naturally occurring mine colonizing plants such Acacia farnesiana, Brickellia coulteri, Baccharis sarothroides, Gnaphalium leucocephalum without finding local adaptation. We explored rhizosphere microbes contributors in both culturable situ diversity rhizospheric bacteria using 16S rRNA gene metagenomic shotgun sequencing. built synthetic (SC) from tailings. SC was then foundation for serial passes experiment grown plant-derived nutrient sources, selecting heavy metals tolerance, cooperation, competition. outcome named ‘final community’ (FSC). Overall, decreased uncultivable roots (399 genera) to cultivated communities (291 genera), (94 lowest FSC (43 genera). Metagenomic clustered into 94,245 protein families, where found growth promotion-related genes csgBAC entCEBAH, coded metagenome-assembled genome Kosakonia sp. Nacozari. Finally, used inoculate greenhouse experiment. with inocula observed higher relative rates sterile substrates. presents promising features might make it useful phytostabilization tailored strategies.