Metabolic homoeostasis of l-threonine in the normally-fed rat. Importance of liver threonine dehydrogenase activity
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چکیده
منابع مشابه
Metabolic homoeostasis of L-threonine in the normally-fed rat. Importance of liver threonine dehydrogenase activity.
Threonine dehydratase, threonine aldolase and threonine dehydrogenase activities were assayed in livers of rats that had been normally-fed, starved for 72 h, fed a high-protein diet or normally-fed and injected with glucagon or cortisone. A modified continuous spectrophotometric assay for threonine aldolase overcame interference resulting from threonine dehydratase activity and revealed that th...
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15 صفحه اولL-Threonine dehydrogenase of Escherichia coli K-12.
A rapid method for purifying L-threonine dehydrogenase from Escherichia coli K-12 cells, grown on a medium containing L_-threonine as carbon source, has been developed. The procedure consists of three fractionation steps of which the last is adsorption and elution of the enzyme from a column of Blue dextran-Sepharose. Homogeneous and stable samples of the dehydrogenase are obtained. L-Threonine...
متن کاملL-Threonine dehydrogenase of chicken liver. Purification, characterization, and physiological significance.
L-Threonine dehydrogenase (L-threonine:NAD+ oxidoreductase (EC 1.1.1.103) has been purified to apparent homogeneity from chicken liver mitochondria. The presence of 2-mercaptoethanol and glycerol is necessary for stabilizing the enzyme during purification and storage. The enzyme is a monomer and has Mr approximately equal to 88,000. The pH optimum is 8.6 to 8.7, and the isoelectric point of the...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1983
ISSN: 0264-6021
DOI: 10.1042/bj2140687