Metabolic Difference Analysis of Clostridium cellulovorans Grown on Glucose and Cellulose

As an anaerobic butyrate-producing bacterium, Clostridium cellulovorans can secrete a variety of extracellular enzymes to degrade plant-based cellulose. However, with glucose as the carbon source, it still secretes large amount protein in broth. The metabolism and regulation are obscure need be further studied. Hence, this study, C. was used conduct fed-batch fermentation microcrystalline at pH 7.0 produce higher level butyrate bioreactor. It produced 16.8 mM lactate, 22.3 acetate, 132.7 72 h during fermentation. In contrast, only 11.5 acetate 93.9 took 192 complete cellulose source. Furthermore, there no lactate detected analysis source balance redox showed that 57% consumed form acids fermentation, while 47% for acid generation Meanwhile, broth both (0.9 ± 0.1 g/L) (1.1 0.2 These results also main product. metabolized generate intermediate metabolites reducing powers (NADH Fdred), then synthesis power maintain cell metabolism. comparative transcriptomics proteomics supported above conclusion. method studying species provides new perspective study on metabolic regulation.