Messenger RNA for Globin in the Postribosomal Supernatant of Rabbit Reticulocytes
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چکیده
منابع مشابه
Messenger RNA for globin in the postribosomal supernatant of rabbit reticulocytes.
10S RNA has been isolated from the postribosomal supernatant of rabbit reticulocyte lysate. This RNA is associated with protein in a ribonucleoprotein particle that sediments at approximately 20S. The 10S RNA extracted from this particle codes predominantly for alpha globin chains when it is translated by a mouse ascites cell-free extract. The product of the cell-free synthesis has been charact...
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both give rise to two additional fragments six and seven bases long with the smaller fragment existing in twice the quantity of the larger one. The individual peaks appear on the chromatograph in the same position if re-run on DEAE-Sephadex or after a second digestion with pancreatic RNAase. This finding indicates that each fragment from the RNAase TI digestion is composed of two different olig...
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Fertilized mouse ova were injected with messenger RNA for rabbit globin. The ova were labelled with [H]leucine and the synthesized rabbit a-globin measured by immunoprecipitation. Injection of rabbit globin mRNA from 4-0 to 15-8pg/ovum resulted in the synthesis of ~1200 dpm/ovum of a-globin during an 18 h incubation. A concentration of mRNA that was translated below the maximum level of globin ...
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S1 nuclease isolated from Aspergillus oryzae has been used to investigate the secondary structure of rabbit globin messenger RNA (mRNA). The enzyme, which is specific for single stranded nucleotides, digests globin mRNA to a limited extent, with 65-75% of the mRNA nucleotides resistant to digestion under mild conditions. This limited digestion is not due to enzyme inactivation, but rather to th...
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Functional messenger RNA (mRNA) for human globin synthesis was isolated from reticulocytes of each of two patients with hemoglobin H disease. The RNA was tested for its capacity to direct globin synthesis in a messenger RNA-dependent cell-free system derived from Krebs Type II mouse ascites tumor cells. In each case, hemoglobin H disease mRNA directed the synthesis of a great excess of /3-globi...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1972
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.69.6.1425