Membrane-bound Conformation of M13 Major Coat Protein
نویسندگان
چکیده
منابع مشابه
The Membrane-Bound Form of Gene 9 Minor Coat Protein of Bacteriophage M13
eindelijk die kast opruimen!). Summary 108 Samenvatting 111 Curriculum vitae 115 List of Publications 117 Het in dit proefschrift beschreven onderzoek werd mogelijk gemaakt met financiële steun van de Stichting Levenswetenschappen (SLW) van de Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO). Abbreviations ATR-FTIR attenuated total reflection Fourier transform infrared CD circular ...
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We have reported variants of the M13 bacteriophage major coat protein (P8) that enable high copy display of monomeric and oligomeric proteins, such as human growth hormone and steptavidin, on the surface of phage particles (Sidhu SS, Weiss GA, Wells JA. 2000. High copy display of large proteins on phage for functional selections. J Mol Biol 296:487-495). Here, we explore how an optimized P8 var...
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Quantification of lipid selectivity by membrane proteins has been previously addressed mainly from electron spin resonance studies. We present here a new methodology for quantification of protein-lipid selectivity based on fluorescence resonance energy transfer. A mutant of M13 major coat protein was labeled with 7-diethylamino-3((4'iodoacetyl)amino)phenyl-4-methylcoumarin to be used as the don...
متن کاملConstrained modeling of spin-labeled major coat protein mutants from M13 bacteriophage in a phospholipid bilayer.
The family of three-dimensional molecular structures of the major coat protein from the M13 bacteriophage, which was determined in detergent micelles by NMR methods, has been analyzed by constrained geometry optimization in a phospholipid environment. A single-layer solvation shell of dioleoyl phosphatidylcholine lipids was built around the protein, after replacing single residues by cysteines ...
متن کاملA coat protein of the bacteriophage M13 virion participates in membrane-oriented synthesis of DNA.
Several molecules of a protein specified by gene 3 of M13 comprise a minor fraction of the phage coat and have been assigned a role in adsorption to the bacterial cell. We find that the gene-3 protein molecules of the virion are fully conserved in phage that have attached irreversibly to the host cell, and they form a complex with the phage DNA when it has been converted to a duplex replicative...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2005
ISSN: 0021-9258
DOI: 10.1074/jbc.m505875200