Melatonin pretreatment can improve the therapeutic effect of adipose-derived stem cells on CCl₄-induced liver fibrosis

In this study, the therapeutic effect of Mel-incubated Adipose-derived mesenchymal stem cells (ADSCs) on CCl4-induced hepatic fibrosis was investigated. Mice with were intraperitoneally injected 8% CCl4 twice a week for 4 weeks. Starting from 5, mice in ADSC group and + Mel 1 mL PBS cell suspension containing × 106 ADSCs or pretreated 50 nM 2 consecutive Results: model group, severe histopathological changes observed liver, including vacuolation, nuclear fragmentation, liver fibrosis, these ameliorated by Mel-pretreated ADSCs. RT-qPCR results showed that Mel-induced significantly inhibited expression proapoptotic genes (Caspase-8, Bax, Caspase-3) promoted an anti-apoptotic gene (Bcl-2). Immunohistochemical large number MMP-9-, TGF-β-, MMP-2-positive present tissues while positive reduced ELISA also levels TNF-α IL-6. Conclusion: improved fibrosis.