Medium composition potentially regulates the anthocyanin production from suspension culture of Daucus carota
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چکیده
منابع مشابه
Elicitation of anthocyanin production in callus cultures of Daucus carota and involvement of calcium channel modulators
The effect of fungal elicitors and calcium-channel modulators on the anthocyanin production in callus cultures of Daucus carota was studied. It was found that the addition of fungal elicitors resulted in higher anthocyanin accumulation on the 3rd day of culture, but they resulted in lower levels of anthocyanin on the 21st day. Fungal elicitor treatment also affected growth of the cultures, as e...
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A reliable procedure for the isolation o f vacuoles from anthocyanin-containing cells o f Daucus carota cell suspension cultures has been developed. From cells o f the late linear growth phase, protoplasts were prepared and purified in a sucrose/sorbitol gradient. Vacuoles were liberated from these protoplasts by osmotic shock and purified in a Metrizamide step gradient. The vacuolecontaining f...
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Embryogenic callus culture of carrot (Daucus carota L.) was induced from the hypocotyl explants in MS medium supplemented with Casein hydrolysate, 2,4-D and BAP for somatic embryogenesis In vitro cell suspension cultures for high multiplication rate. Embryogenic callus was transferred to liquid basal Murashige and Skoog medium The mature somatic embryos were encapsulated in a Sodium alginate mi...
متن کاملCharacterization and Properties of Different Glucosyltransferases Isolated from Suspension-Cultured Cells of Daucus carota
Particulate enzymes (14,000 g pellet) from suspension-cultured carrot cells (Daucus carota L .) incorporated glucose from UDP-glucose and G DP-glucose into ethanol-insoluble products which were characterized as glucans or glucoprotein. Based on the test system to assay glucansynthetases I and II four different enzymatic activities could be distinguished on the basis o f their substrate and diva...
متن کاملPurification and Interconversion of Homoserine Dehydrogenase from Daucus carota Cell Suspension Cultures.
Homoserine dehydrogenase from cell suspension cultures of carrot (Daucus carota L.) has been purified to apparent homogeneity by a combination of selective heat denaturation, ion exchange and gel filtration chromatographies, and preparative gel electrophoresis. Carrot homoserine dehydrogenase is composed of subunits of equal molecular weight (85,000 +/- 5,000). During purification, the enzyme e...
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ژورنال
عنوان ژورنال: 3 Biotech
سال: 2018
ISSN: 2190-572X,2190-5738
DOI: 10.1007/s13205-018-1146-x