Mediating Ribosomal Competition by Splitting Pools

Synthetic biology constructs often rely upon the introduction of “circuit” genes into host cells, in order to express novel proteins and thus endow with a desired behavior. The expression these new “consumes” existing resources cell, such as ATP, RNA polymerase, amino acids, ribosomes. Ribosomal competition among strands mRNA may be described by system nonlinear ODEs called Flow Model (RFM). for between circuit can ameliorated splitting ribosome pool use orthogonal ribosomes, where are exclusively translated mutated In this letter, RFM is extended include competition. This Orthogonal (ORFM) proven stable through Robust Lyapunov Functions. optimization problem maximizing weighted protein translation rate adjusting allocation ribosomal species formulated.