Median lethal Dose and Acute Toxicity of Rosa canina L: In-Vivo Study

Rosa canina belongs to rosacea family. Rosa. has a high concentration of phyto-constituents such as flavonoids, carotenoids, triterpene and vitamins vitamin C, E, A have an anti-inflammatory antioxidant
 
 effects. The antioxidant effect presence large quantities phytochemicals flavonoids polyphenols. been used for arthritis gout, osteoarthritis, urinary tract disorder, diabetes, inflammation cancer. After grinding the leaves canina, they are extracted by using ethanol solvent (cold extraction method), then extract concentrated rotary evaporator at 40 c° leaving it dry. Median lethal dose (LD50) examined on 84 mice (male female) divided into seven groups, each one contains 12 (6 male 6 female). animals were monitored signs any behavior changes after administration extract. Acute toxicity study was done 20 rats female fourteen days. weight taken day 0, 7, 14. At fourteen, relative organ well histopathological examination (heart, liver, spleen, kidney, lung, abdominal stomach, testes ovaries) taken. In addition serum biochemical tests for) blood glucose, urea, creatinine, ALT, AST total bilirubin (were fourteen. result this study, indicated that 50 16.527 gram/kilogram. revealed there is no significant difference between controlled treated groups all organs selected mentioned above. addition, both groups. Finally, found regarding histopathology due p value P ˃ 0.05.
 Conclusion
 According presented showed wide range safety depending 50h (16.527 g/kg). Therefore, considered nontoxic. No cytotoxic appeared in acute study. This results obtained, which include control biochemistry examination.