Media Optimization by Response Surface Methodology for the Enhanced Production of Acidic Extracellular Pectinase by the Indigenously Isolated Novel Strain Aspergillus cervinus ARS2 Using Solid-State Fermentation

Pectinolytic enzymes are related that hydrolyze pectic substances. of great interest in industrial applications for softening fruits, extracting and clarifying juices, olive oil, retting textile fibers, preparing gel, isolating protoplasts. The current work presents acidic extracellular pectinase production using low-cost agro-industrial waste with the indigenously isolated novel strain Aspergillus cervinus. Two fungal isolates, ARS2 ARS8, maximum activity, 41.88 ± 1.57 IU/mL 39.27 1.14 IU/mL, respectively, were screened out 27 isolates from decayed fruit peels (orange, banana, lemon) soil containing decomposed vegetables. isolate ARS2, identified as cervinus by molecular characterization, showed highest activity 43.05 1.38IU/mL during screening was further used media component optimization studies. To understand their effect on one-factor-at-a-time (OFAT) studies conducted carbon sources, nitrogen mineral salts. OFAT results orange peel (carbon source) at 44.51 1.33 peptone (nitrogen 45.05 1.04 NaH2PO4 (mineral salts) 43.21 1.12 IU/mL. most significant components Plackett–Burman (PB) design based p-value, Pareto chart, main plot, (p < 0.001), KH2PO4 which optimized Response Surface Methodology (RSM) Central Composite Design (CCD). a 105.65 0.31 10.63 g peel, 3.96 g/L peptone, 2.07 KH2PO4, 2.10 NaH2PO4. Thus, it discovered able to successfully produce amount waste. Therefore, can be considered large-scale meet demands.