Mechanisms of Replication-Deficient Vaccinia Virus/T7 RNA Polymerase Hybrid Expression: Effect of T7 RNA Polymerase Levels and α-Amanitin
نویسندگان
چکیده
منابع مشابه
Use of a hybrid vaccinia virus-T7 RNA polymerase system for expression of target genes.
A novel expression system based on coinfection of cells with two recombinant vaccinia viruses has been developed. One recombinant vaccinia virus contained the bacteriophage T7 RNA polymerase gene under control of a vaccinia virus promoter. The second recombinant vaccinia virus contained a target gene of choice flanked by bacteriophage T7 promoter and termination sequences. Maximum expression of...
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Recent models of RNA polymerase transcription complexes have invoked the idea that enzyme-nascent RNA contacts contribute to the stability of the complexes. Although much progress on this topic has been made with the multisubunit Escherichia coli RNA polymerase, there is a paucity of information regarding the structure of single-subunit phage RNA polymerase transcription complexes. Here, we pho...
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The late regions of the T7 and T3 bacteriophage genomes are transcribed by phage-specified RNA polymerases, the products of gene 1. Although these phage transcriptional systems share many characteristics and are obviously related, they have diverged to such an extent that neither of their respective RNA polymerases utilizes the promotor sites of the other phage at an appreciable rate. However, ...
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The high specificity of T7 RNA polymerase (RNAP) for its promoter sequence is mediated, in part, by a specificity loop (residues 742-773) that projects into the DNA binding cleft (1). Previous work demonstrated a role for the amino acid residue at position 748 (N748) in this loop in discrimination of the base pairs (bp) at positions -10 and -11 (2). A comparison of the sequences of other phage ...
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ژورنال
عنوان ژورنال: Virology
سال: 1998
ISSN: 0042-6822
DOI: 10.1006/viro.1998.9070