Mechanism of Aconitase Action

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Mechanism of Aconitase Action

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The Mechanism of Aconitase Action

When a number of metal ions were tested as activators over a lOO-fold range of metal ion concentration (0.01 to 1.0 mM), aconitase was specifically activated by Fe(II). Manganese(I1) does not activate aconitase but binds to the enzyme, as shown indirectly by inhibition of the enzyme activation by Fe(I1) and directly by electron paramagnetic resonance. A titration of the enzyme with Mn(I1) may b...

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The Mechanism of Aconitase Action II. MAGNETIC RESONANCE STUDIES OF THE COMPLEXES

When a number of metal ions were tested as activators over a lOO-fold range of metal ion concentration (0.01 to 1.0 mM), aconitase was specifically activated by Fe(II). Manganese(I1) does not activate aconitase but binds to the enzyme, as shown indirectly by inhibition of the enzyme activation by Fe(I1) and directly by electron paramagnetic resonance. A titration of the enzyme with Mn(I1) may b...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1967

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)96082-5