Measurements of Protein-Protein Interactions by Size Exclusion Chromatography
نویسندگان
چکیده
منابع مشابه
Measurements of protein-protein interactions by size exclusion chromatography.
A method is presented for determining second virial coefficients (B(2)) of protein solutions from retention time measurements in size exclusion chromatography. We determine B(2) by analyzing the concentration dependence of the chromatographic partition coefficient. We show the ability of this method to track the evolution of B(2) from positive to negative values in lysozyme and bovine serum alb...
متن کاملA Deadenylase Assay by Size-Exclusion Chromatography
The shortening of the 3'-end poly(A) tail, also called deadenylation, is crucial to the regulation of mRNA processing, transportation, translation and degradation. The deadenylation process is achieved by deadenylases, which specifically catalyze the removal of the poly(A) tail at the 3'-end of eukaryotic mRNAs and release 5'-AMP as the product. To achieve their physiological functions, all dea...
متن کاملPolymer Separation by Size Exclusion Chromatography
Proteins are the essence of life processes. DNA contains the coded information for life, and is analogous to computer software, but it is the proteins that are analogous to the hardware, that actually carry out the job. Proteins have many roles, from catalysts (enzymes), through proteins that bind and interact with other molecules to control their behaviour, to structural and storage proteins w...
متن کاملOrganic matter from biofilter nitrification by high performance size exclusion chromatography and fluorescence excitation-emission matrix
A combination of high performance size exclusion chromatography with organic carbon detector and ultraviolet detector coupled with peak-fitting technique and fluorescence excitation-emission matrix spectrometry applied fluorescence regional integration method was conducted to determine the characteristics of organic matter during nitrification. The batch scale of bionet nitrification without or...
متن کاملSize Exclusion Chromatography of Polymers
In the characterization of polymers, size-exclusion chromatography (SEC) has become a standard technique for determining molar mass averages andmolar mass distributions (MMD) of polymers. The principle of SEC is easily understood: owing to limited accessability of the pore volume within the particles of the column packing, polymer molecules are separated according to their hydrodynamic volumes,...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2003
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(03)74684-0