Measurement of Triiodothyronine in Urine

[Measurement of triiodothyronine in urine].

A radioimmunoassay procedure to measure triiodothyronine (T3) in unextracted urine is described. One hundred microliter of T3 standards or samples were incubated with 100 microliter of T3 antiserum (1:40,000), 100 microliter of tracer 125I-T3 and 700 microliter of 0.05 M borate buffer, pH 8.6. Separation of free from bound antigen was achieved by dextran coated charcoal. The cross-reaction of L...

Radioimmunoassay for measurement of triiodothyronine in human serum.

A convenient, specific, precise, and reproducible radioimmunoassay system for measurement of triiodothyronine (T(3)) in human serum has been developed. The procedure compares the ability of standards and unknowns to compete with radioactive T(3) for binding sites on a T(3)-binding antiserum produced in rabbits by immunization with human thyroglobulin. The assay is set up in the presence of 250 ...

Measurement of mercury in human urine.

Four methods of determining the concentration of mercury in human urine have been studied. A simple method suitable for general laboratory use is recommended and the requirements for accurate results are defined. The method employs mild oxidation with permanganate and HS(2)O(4) followed by dithizone extraction and measurement of absorbance at 485 nm and 620 nm.No mercury was detected in any of ...

The measurement of thiamine in urine.

The most widely used procedures for the measurement of thiamine in urine are based on the oxidation of the vitamin to thiochrome, which is then extracted into butyl alcohol and measured fluorometrically (1). It has been found possible to omit the butyl alcohol extraction without prejudice to the assay. By utilizing benzenesulfonyl chloride to obtain blank values, as proposed by Urban and Goldma...

Measurement of free light chains in urine.