Measurement of Transmembrane Voltage using the Fluorescent Sensitive Dye Annine-6
نویسندگان
چکیده
منابع مشابه
Ratiometry of transmembrane voltage-sensitive fluorescent dye emission in hearts.
Transmembrane voltage-sensitive fluorescence measurements are limited by baseline drift that can obscure changes in resting membrane potential and by motion artifacts that can obscure repolarization. Voltage-dependent shift of emission wavelengths may allow reduction of drift and motion artifacts by emission ratiometry. We have tested this for action potentials and potassium-induced changes in ...
متن کاملCorrection of motion artifact in transmembrane voltage-sensitive fluorescent dye emission in hearts.
Fast voltage-sensitive dyes are widely used to image cardiac electrical activity. Typically, the emission spectrum of these fluorochromes is wavelength shifted with altered membrane potential, but the optical signals obtained also decay with time and are affected by contraction. Ratiometry reduces, but may not fully remove, these artifacts. An alternate approach has been developed in which the ...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2014
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2013.11.2812