Measurement of Cytosolic Calcium Using 19 F NMR
نویسندگان
چکیده
منابع مشابه
Measurement of cytosolic calcium using 19F NMR.
Fluorine-19 nuclear magnetic resonance (NMR) studies of cells and perfused organs loaded with fluorinated ion chelators represent a new approach to determining cytosolic free calcium levels in situ. The molecular basis for this approach and the relative advantages and disadvantages of the NMR technique are discussed in this paper. Results obtained on perfused normoxic and ischemic rat hearts ar...
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(19)F-NMR has proved to be a valuable tool in fragment-based drug discovery. Its applications include screening libraries of fluorinated fragments, assessing competition among elaborated fragments and identifying the binding poses of promising hits. By observing fluorine in both the ligand and the target protein, useful information can be obtained on not only the binding pose but also the dynam...
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The ion-pair association constant values, related to the reaction + were determined by means of the NMR spectroscopy. The values of the thermodynamic functions of the ion pairing process were calculated on the basis of the NMR results. In addition, the association entropy was found to be dependent on the temperature. Comparing the experimental data and the Fuoss theory it is found that ion-pa...
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ژورنال
عنوان ژورنال: Environmental Health Perspectives
سال: 1990
ISSN: 0091-6765
DOI: 10.2307/3430709