mazFas a counter-selectable marker for unmarked genetic modification ofPichia pastoris
نویسندگان
چکیده
منابع مشابه
mazF, a novel counter-selectable marker for unmarked chromosomal manipulation in Bacillus subtilis
Here, we present a novel method for the directed genetic manipulation of the Bacillus subtilis chromosome free of any selection marker. Our new approach employed the Escherichia coli toxin gene mazF as a counter-selectable marker. The mazF gene was placed under the control of an isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible expression system and associated with a spectomycin-resistanc...
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ژورنال
عنوان ژورنال: FEMS Yeast Research
سال: 2009
ISSN: 1567-1356,1567-1364
DOI: 10.1111/j.1567-1364.2009.00503.x