Matrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry: A Promising Technique for Reproductive Research1

Matrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry

A new matrix assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) method to spatially profile the location and distribution of multiple N-linked glycan species in tissues is described. Application of an endoglycosidase, peptide N-glycosidase F (PNGaseF), directly on tissues followed by incubation releases N-linked glycan species amenable to detection by MALDI-IMS. The meth...

Application of Matrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry

The clarification of metabolic dynamics in lesion areas is important. Many approaches, such as high performance liquid chromatography mass spectrometry, gas chromatography mass spectrometry, immunohistochemistry, are used to define disease-related abnormalities. Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) is attracting attention as a new valuable tool. MALD...

Imaging Matrix Assisted Laser Desorption Ionization Mass Spectrometry: a technique to map plant metabolites within tissues at high spatial resolution.

Imaging Matrix Assisted Laser Desorption Ionization Mass Spectrometry provides a new and powerful tool to analyse the distribution of metabolites within plant tissues. The two matrices alpha-cyano-4-hydroxycinnamic acid (alpha-CHCA) and 9-aminoacridine provide a useful combination that allows the measurement of amino acids, sugars, and phosphorylated metabolites. Results are presented showing t...

Improving tissue preparation for matrix-assisted laser desorption ionization mass spectrometry imaging. Part 1: using microspotting.

Nowadays, matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) is a powerful technique to obtain the distribution of endogenous and exogenous molecules within tissue sections. It can, thus, be used to study the evolution of molecules across different physiological stages in order to find out markers or get knowledge on signaling pathways. In order to provide valuabl...

matrix-assisted laser desorption/ionization time of flight mass spectrometry: a new guide to infectious disease