Mass spectral determination of phosphopantetheinylation specificity for carrier proteins in Mycobacterium tuberculosis
نویسندگان
چکیده
منابع مشابه
Mass spectral determination of phosphopantetheinylation specificity for carrier proteins in Mycobacterium tuberculosis
Phosphopantetheinyl transferases (PPTases) are key elements in the modular syntheses performed by multienzyme systems such as polyketide synthases. PPTases transfer phosphopantetheine derivatives from Coenzyme A to carrier proteins (CPs), thus orchestrating substrate supply. We describe an efficient mass spectrometry-based protocol for determining CP specificity for a particular PPTase in organ...
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15 صفحه اولIn silico Study of Target Proteins for Mycobacterium tuberculosis
The completion of the genome of pathogens and the human has provided data that can be utilized to design vaccines and drug targets. One of the recently adopted strategies for drug designing is based on comparative genomics approach, it gives a set of genes that are likely to be essential to the pathogen but absent in the host. By performing homology searches and structural modeling, we can dete...
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ژورنال
عنوان ژورنال: FEBS Open Bio
سال: 2016
ISSN: 2211-5463
DOI: 10.1002/2211-5463.12140