Marker-Assisted Backcross Breeding for Improving Bruchid (Callosobruchus spp.) Resistance in Mung Bean (Vigna radiata L.)

Mung bean is vulnerable to bruchids (Callosobruchus spp.), resulting in low quality and losses worldwide. Developing resistant cultivars the most effective, economical, eco-friendly way protect mung seeds from damage of bruchids. Previously, we identified two gene loci that are tightly linked with bruchid resistance, which makes new cultivar development possible using molecular assisted selection (MAS). In present study, marker-assisted backcross (MABC) breeding was employed introgress resistance VrPGIP2 locus donor parent V2802 into a popular cultivar, Kamphaeng Saen 1. Markers VrBR-SSR013 DMB-SSR158 were used for foreground selection, phenotypic background selection. Three advanced inbred lines (R67-11, R67-22, R92-15) carrying developed BC3F6 population. Further analysis showed line R67-22 performed best; it highly excellent agronomic traits field. Therefore, could be further evaluated large-scale trials release as variety.