Mapping the dynamic high-density lipoprotein synapse
نویسندگان
چکیده
Background and aimsHeterogeneous high-density lipoprotein (HDL) particles, which can contain hundreds of proteins, affect human health disease through dynamic molecular interactions with cell surface proteins. How HDL mediates its long-range signaling functions various types is largely unknown. Due to the complexity HDL, we hypothesize that multiple receptors engage particles resulting in condition-dependent receptor-HDL interaction clusters at surface.MethodsHere used mass spectrometry-based light-controlled proximity labeling strategy LUX-MS a discovery-driven manner decode HDL-receptor interactions.ResultsSurfaceome nanoscale organization analysis hepatocytes endothelial cells using revealed previously known HDL-binding protein scavenger receptor B1 (SCRB1) embedded community, term synapse. Modulating synapse, composed 60 by silencing individual members showed synapse be assembled absence SCRB1 are interlinked. The aminopeptidase N (AMPN) (also as CD13) was identified an member directly influences uptake into primary aortic (HAECs).ConclusionsOur data indicate preformed residing complexes modulate function suggest new theragnostic opportunities.
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ژورنال
عنوان ژورنال: Atherosclerosis
سال: 2023
ISSN: ['0021-9150', '1879-1484']
DOI: https://doi.org/10.1016/j.atherosclerosis.2023.117200