Mapping of immunoglobulin variable region genes: relationship to the ‘deletion’ model of immunoglobulin gene rearrangement
نویسندگان
چکیده
منابع مشابه
Evolution of immunoglobulin kappa chain variable region genes in vertebrates.
The major source of immunoglobulin diversity is variation in DNA sequence among multiple copies of variable region (V) genes of the heavy- and light-chain multigene families. In order to clarify the evolutionary pattern of the multigene family of immunoglobulin light kappa chain V region (V kappa) genes, phylogenetic analyses of V kappa genes from humans and other vertebrate species were conduc...
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We have tested whether immunoglobulin light chain variable region genes are capable of directing initiation of transcription without undergoing the DNA rearrangement which creates a complete immunoglobulin gene. Two human Vk genes specifically initiated transcription in vitro at a site which is approximately 30 bases downstream of a TATA box and 20 bases upstream of the initiation codon. A Vk p...
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We have assessed the number of times the gene sequence encoding constant regions of mouse immunoglobulin heavy chains gamma1, gamma2a, and gamma3 are represented in the mouse genome by hybridization kinetic analysis. All three genes are present at one copy each per haploid genome in normal tissues and myelomas producing IgM or IgG3. IgG1-producing myelomas, however, contain 1 copy each of the g...
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Immunoglobulin genes are generated during differentiation of B lymphocytes by joining gene segments. A mouse pre-B cell contains a functional immunoglobulin heavy-chain gene, but no light-chain gene. Although there is only one heavy-chain locus, there are two light-chain loci: kappa and lambda. It has been reported that kappa loci in the germ-line configuration are never (in man) or very rarely...
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We have investigated the genetic origin of autoantibody production in several strains of mice that spontaneously develop a systemic lupus erythematosus-like disease. Restriction fragment length polymorphism analyses of gene loci encoding kappa light chain variable regions (Igk-V) demonstrated, as shown previously for the Ig heavy chain locus, that autoantibody production and disease occur in di...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1981
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/9.21.5725