Map of the IncP1β Plasmid pTSA Encoding the Widespread Genes ( tsa ) for p- Toluenesulfonate Degradation in Comamonas testosteroni T-2
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چکیده
منابع مشابه
Map of the IncP1beta plasmid pTSA encoding the widespread genes (tsa) for p-toluenesulfonate degradation in Comamonas testosteroni T-2.
The catabolic IncP1beta plasmid pTSA from Comamonas testosteroni T-2 was mapped by subtractive analysis of restriction digests, by sequencing outwards from the tsa operon (toluenesulfonate degradation), and by generating overlapping, long-distance-PCR amplification products. The plasmid was estimated to comprise 72 +/- 4 kb. The tsa region was found to be a composite transposon flanked by two I...
متن کاملEnviron . Microbiol . Tewes Tralau , Alasdair M . Cook and Juergen Ruff T - 2 Comamonas testosteroni Toluenesulfonate Degradation in p - ) for tsa the Widespread Genes ( Plasmid pTSA Encoding
Toluenesulfonate Degradation in p-) for tsa the Widespread Genes (Plasmid pTSA Encoding β Map of the IncP1
متن کاملCharacterization of TsaR, an oxygen-sensitive LysR-type regulator for the degradation of p-toluenesulfonate in Comamonas testosteroni T-2.
TsaR is the putative LysR-type regulator of the tsa operon (tsaMBCD) which encodes the first steps in the degradation of p-toluenesulfonate (TSA) in Comamonas testosteroni T-2. Transposon mutagenesis was used to knock out tsaR. The resulting mutant lacked the ability to grow with TSA and p-toluenecarboxylate (TCA). Reintroduction of tsaR in trans on an expression vector reconstituted growth wit...
متن کاملCharacterization of the p-toluenesulfonate operon tsaMBCD and tsaR in Comamonas testosteroni T-2.
Comamonas testosteroni T-2 uses a standard, if seldom examined, attack on an aromatic compound and oxygenates the side chain of p-toluenesulfonate (TS) (or p-toluenecarboxylate) to p-sulfobenzoate (or terephthalate) prior to complete oxidation. The expression of the first three catabolic enzymes in the pathway, the TS methyl-monooxygenase system (comprising reductase B and oxygenase M; TsaMB), ...
متن کاملConjugative plasmids and the degradation of arylsulfonates in Comamonas testosteroni.
Comamonas testosteroni T-2 degrades p-toluenesulfonate (TSA) via p-sulfobenzoate (PSB) and protocatechuate and degrades toluenecarboxylate via terephthalate (TER) and protocatechuate. The appropriate genes are expressed in at least five regulatory units, some of which are also found in C. testosteroni PSB-4 (F. Junker, R. Kiewitz, and A. M. Cook, J. Bacteriol. 179:919-927, 1997). C. testosteron...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2001
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.67.4.1508-1516.2001