منابع مشابه
Analysis of glycosaminoglycans in stem cell glycomics.
Glycosaminoglycans (GAGs) play a critical role in the binding and activation of growth factors in cell signal transduction required for biological development. A glycomics approach can be used to examine GAG content, composition, and structure in stem cells in order to characterize their general differentiation. Specifically, this method may be used to evaluate chondrogenic differentiations by ...
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Glycomics-an integrated approach to study structure-function relationships of complex carbohydrates (or glycans)-is an emerging field in this age of post-genomics. Realizing the importance of glycomics, many large scale research initiatives have been established to generate novel resources and technologies to advance glycomics. These initiatives are generating and cataloging diverse data sets n...
متن کاملGlycomics of proteoglycan biosynthesis in murine embryonic stem cell differentiation.
Glycosaminoglycans (GAGs) play a critical role in binding and activation of growth factors involved in cell signaling critical for developmental biology. The biosynthetic pathways for GAGs have been elucidated over the past decade and now analytical methodology makes it possible to determine GAG composition in as few as 10 million cells. A glycomics approach was used to examine GAG content, com...
متن کاملGlycomics Using Mass Spectrometry
Mass spectrometry (MS), which has advanced rapidly in recent years, is expected to become the tool of choice for high-throughput analysis of relationships between structures and functions of glycans. The structural analysis of glycans using MS employs a number of methods for pretreatment, derivatization, and interpretation. However, since none of these are universally applicable, researchers ha...
متن کاملCell-Based Microscale Isolation of Glycoaminoglycans for Glycomics Study.
Glycomics research requires the isolation of glycans from cells for structural characterization and functional studies of the glycans. A method for cell-based microscale isolation and quantification of highly sulfated, moderately sulfated, and nonsulfated glycosaminoglycans (GAGs) was developed using Chinese hamster ovary (CHO) cells. This microscale isolation relies on a mini-strong anion exch...
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ژورنال
عنوان ژورنال: Journal of Glycomics & Lipidomics
سال: 2013
ISSN: 2153-0637
DOI: 10.4172/2153-0637.s5-e001