Malachite green-INT (MINT) method for determining active bacteria in sewage

Use of malachite green for staining flagella in bacteria.

Preparations are prepared, using completely clean slides and aqueous suspensions of young bacterial agar cultures. A drop of the suspension is placed on one end of the slide; the slide is then tipped, making the suspension drain down to the opposite end (Society of American Bacteriologists, Manual of Microbiological Methods, McGraw-Hill Book Co., New York, 1957). Both the mordanting and the sta...

Reduction of malachite green to leucomalachite green by intestinal bacteria.

Intestinal microfloras from human, rat, mouse, and monkey fecal samples and 14 pure cultures of anaerobic bacteria representative of those found in the human gastrointestinal tract metabolized the triphenylmethane dye malachite green to leucomalachite green. The reduction of malachite green to the leuco derivative suggests that intestinal microflora could play an important role in the metabolic...

Risk Assessment of Malachite Green in Food

A malachite green colorimetric assay for protein phosphatase activity.

A simple and sensitive colorimetric assay for protein phosphatase activity based on the determination of released Pi by an improved malachite green procedure (A. A. Baykov, O. A. Evtushenko, and S.M. Avaeva, 1988, Anal. Biochem. 171, 266-270) is described. Proteins must be removed or stabilized prior to Pi determination with 0.25 N sulfuric acid or 3% (w/w) perchloric acid. Alternatively, to av...

Improved automated method for determining serum albumin with bromcresol green.

An automated method for determining serum albumin with bromcresol green according to Doumas et al. [C/in. Chim. Acta 31, 87 (1971)] was simplified and improved for use in routine analysis. The manifold is simpler, there is better sample separation and less sample interaction, both within-run and day-to-day run precision are excellent, and results agree well with those obtained by electrophoresi...