Making Silianted Slides for Mounting Sections
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چکیده
منابع مشابه
Mounting live cells onto microscope slides.
This protocol describes the mounting of cells which have been grown on cleaned coverslips in cell culture dishes containing the appropriate medium and supplements. Labeling is performed with the coverslips placed in culture dishes. After cells are labeled, the coverslips can be mounted onto microscope slides to be viewed for short-term observation of live cells or longer-term observation of fix...
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BACKGROUND Laser microbeam microdissection has greatly facilitated the procurement of specific cell populations from tissue sections. However, the fact that a coverslip is not used means that the morphology of the tissue sections is often poor. AIMS To develop a mounting method that greatly improves the morphological quality of tissue sections for laser microbeam microdissection purposes so t...
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A considerable need has been felt for a simple and reliable method of mounting serial sections for electron microscopy. Techniques so far published have necessitated special optical and micromanipulative equipment. An extremely simple and inexpensive micromanipulator capable of very fine movements in three dimensions was first described by Goldacre (1954). It consisted of several glass microsco...
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D u r i n g the d e v e l o p m e n t of n e w m e t h o d s for p re p a r i n g u l t r a t h i n sect ions of u n e m b e d d e d t issue for e lec t ron m i c r o s c o p y (1, 2, 3, 5), t h e p r o b l e m c a m e u p of p l a c i n g the sect ions o n t h e gr id. M a n y exp e r i m e n t s s h o w e d t h a t f loa t ing t h e sect ions on w a t e r cou ld resu l t in n u m e r o u s pe...
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ژورنال
عنوان ژورنال: Microscopy Today
سال: 1998
ISSN: 1551-9295,2150-3583
DOI: 10.1017/s1551929500059083