Making Colony PCR Easier by Adding Gel-Loading Buffer to the Amplification Reaction
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چکیده
منابع مشابه
Making colony PCR easier by adding gel-loading buffer to the amplification reaction.
Because of its simplicity, PCR performed directly from bacterial colonies on agar plates has been extensively used as a first step in the identification of recombinant clones (1–3,6,7). Instead of making DNA minipreps and restriction digestion from several putative recombinant colonies, colony PCR allows the identification of the positive clones, and so reduces these procedures to one clone per...
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of DNA melting curves during the polymerase chain reaction. Anal. Biochem. 245:154-160. 7.Trka, J., V. Divoky, and T. Lion. 1995. Prevention of product carry-over by single tube two-round (ST-2R) PCR: application to BCRABL analysis in chronic myelogenous leukemia. Nucleic Acids Res. 23:4736-4737. 8.Wittwer, C.T., K.M. Ririe, R.V. Andrew, D.A. David, R.A. Gundry and U.J. Balis. 1997. The LightCy...
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Pipet tip loading of polymerase chain reaction (PCR) and other amplification products into an electrophoresis gel represents a potential source of laboratory contamination. We have developed a prototype of the gel contamination control system (GelCCS) that enables gel loading by bottom puncture of PCR tubes. Puncture occurs within a sealed gel casing, preventing contamination of the surrounding...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2000
ISSN: 0736-6205,1940-9818
DOI: 10.2144/00283bm06