Major outer membrane proteindofEscherichia coliK 12
نویسندگان
چکیده
منابع مشابه
Genetic locus (ompB) affecting a major outer-membrane protein in Escherichia coli K-12.
Three multiply colicin-tolerant mutants in Escherichia coli K-12 from the TolIV, TolXIV, and TolXV phenotypic groups, all lacking or having only trace amounts of protein 1, a major outer-membrane protein, were mapped by Hfr crosses, and the position on the chromosome was confirmed by cotransduction with nearby markers. The mutations were located near malQP in the 74-min region of the E. coli ch...
متن کاملPrimary structure of major outer membrane protein II (ompA protein) of Escherichia coli K-12.
The amino acid sequence of major outer membrane protein II (ompA protein) from Escherichia coli K-12 has been determined. The transmembrane polypeptide consists of 325 residues, resulting in a molecular weight of 35,159. The transmembrane part of the protein is located between residues 1 and 177. In this part of the protein a predominantly lipophilic 27-residue segment exists that perhaps spans...
متن کاملISOLATION AND PURIFICATION OF MAJOR OUTER MEMBRANE PROTEINS FROM BRUCELLA ABORTUS S-99
Isolation and purification of major outer membrane proteins (OMP) from the cell wall envelope of Brucella abortus S-99 were achieved by sonication, solubilization and membrane fractionation in the presence of non-ionic detergent (Tx-100) and lysozyme treatments, followed by ultracentrifugation. The crude OMP was treated with trypsin to free the preparation from any other protein contaminan...
متن کاملIsolation of the outer membrane and characterization of the major outer membrane protein from Spirochaeta aurantia.
The outer membrane of Spirochaeta aurantia was isolated after cells were extracted with sodium lauryl sarcosinate and was subsequently purified by differential centrifugation and KBr isopycnic gradient centrifugation. The purified outer membrane was obtained in the form of carotenoid-containing vesicles. Four protein species with apparent molecular weights of 26,000 (26K), 36.5K, 41K, and 48.5K...
متن کاملUsing Recombinant Chlamydia Major Outer Membrane Protein (MOMP) in ELISA Diagnostic Kit
Chlamydia trachomatis is one of the main causes of Sexually Transmitted Diseases (STDs) such as prostatitis and epididymitis in men and cervicitis, endometriosis, vaginitis and ureogenital tract infections in women. Serological tests with sensitivities related to specific antigens are commonly used as routine laboratory tests for diagnosis of Chlamydia. In this research the Chlamydia Major O...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1977
ISSN: 0014-5793
DOI: 10.1016/0014-5793(77)80104-x