Magnitude and location of surface charges on Myxicola giant axons.

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Magnitude and location of surface charges on Myxicola giant axons

The effects of changes in the concentration of calcium in solutions bathing Myxicola giant axons on the voltage dependence of sodium and potassium conductance and on the instantaneous sodium and potassium current-voltage relations have been measured. The sodium conductance-voltage relation is shifted along the voltage axis by 13 mV in the hyperpolarizing direction for a fourfold decrease in cal...

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Leak Current Rectification in Myxicola Giant Axons

Early leak current, i.e. for times similar to the time to peak of the transient current was measured in Myxicola giant axons in the presence of tetrodotoxin. The leak current-voltage relation rectifies, showing more current for strong depolarizing pulses than expected from symmetry around the holding potential. A satisfactory practical approximation for most leak corrections is constant resting...

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Current Separations in Myxicola Giant Axons

The effect of reducing the external sodium concentration, [Na](o), on resting potential, action potential, membrane current, and transient current reversal potential in Myxicola giant axons was studied. Tris chloride was used as a substitute for NaCl. Preliminary experiments were carried out to insure that the effect of Tris substitution could be attributed entirely to the reduction in [Na](o)....

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Current- and Voltage-Clamped Studies on Myxicola Giant Axons

A new dissection procedure for preparing Myxicola giant axons for observation under voltage clamp is described. Preparation time is generally 40-45 min. 65-70% of the preparations attempted may be brought through the entire procedure, including insertion of the long internal electrode, and support an initial action potential amplitude of 100 mv or greater. Mean values for axon diameter, resting...

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Sodium efflux in Myxicola giant axons

Several properties of the Na pump in giant axons from the marine annelid Myxicola infundibulum have been determined in an attempt to characterize this preparation for membrane transport studies. Both NaO and KO activated the Na pump of normal microinjected Myxicola axons. In this preparation, the KO activation was less and the NaO activation much greater than that found in the squid giant axon....

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ژورنال

عنوان ژورنال: Journal of General Physiology

سال: 1975

ISSN: 0022-1295,1540-7748

DOI: 10.1085/jgp.66.1.47