Magnetic Activated Cell Sorting (MACS) in egg donation cycles and high sperm DNA fragmentation
نویسندگان
چکیده
منابع مشابه
Reduction of sperm DNA fragmentation using the magnetic activated cell sorting (MACS) with annexin V microbeads in assisted reproduction
Sperm DNA integrity is an important paternal factor that has been shown to influence fertilization rates, embryo development and pregnancy rates. To reduces the number of apoptotic spermatozoa many technics are used before ICSI treatment ( e.g. using of antioxidants, testicular spermatozoa). Recently, the use of annexin V microbeads, as a non-invasive method to reduce high levels of sperm apopt...
متن کاملMagnetic activated cell sorting (MACS): utility in assisted reproduction.
Assisted reproductive techniques (ART) have now been extensively incorporated in the management of infertile couples. But even after rapid methodological and technological advances the success rates of these procedures have been below expectations. This has led to development of many sperm preparation protocols to obtain an ideal semen sample for artificial reproduction. Sperm apoptosis has bee...
متن کاملP-20: Efficiency of Magnetic-Activated Cell Sorting System in Separation of Normal Sperm
Background Sperm preparation before assisted reproductive techniques is very important for obtaining successful fertilization and pregnancy. During ICSI technique, sperms are selected based on viability and morphology. But this selection did not guarantee integrity of sperm DNA. Therefore, novel sperm selection procedures are introduced for selection of sperm with low DNA damage. One of these p...
متن کاملFirst Pregnancy, Somatic and Psychological Status of a 4-Year-Old Child Born following Annexin V TESA Sperm Separation
Introduction Sperm DNA integrity is a crucial paternal factor affecting fertilization and pregnancy rates, as well as embryo development. Case The present case report describes the successful pregnancy after testicular sperm aspiration (TESA) combined with intracytoplasmic sperm injection (ICSI) (TESA-ICSI) in a couple where the male presented high sperm DNA fragmentation. In order to sort dama...
متن کاملThe effect of rhFSH on Sperm DNA Fragmentation and sperm parameters in Oligozoospermia Infertile Men
Background: One of the main causes of male infertility is the negative effects of oxidative stress. Follicle-stimulating hormone (FSH) plays an essential role in spermatogenesis, as well as in the maintenance of sperm DNA integrity. This study aimed to determine whether the recombinant human follicle-stimulating hormone (rhFSH) treatment of sperm parameters could positively affect sperm DNA and...
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ژورنال
عنوان ژورنال: Reproductive BioMedicine Online
سال: 2014
ISSN: 1472-6483
DOI: 10.1016/s1472-6483(14)50057-0