Maceration of Plant Tissues by Pectin trans-Eliminase
نویسندگان
چکیده
منابع مشابه
Eliminative Cleavage of Pectin and of Oligogalacturonide Methyl Esters by Pectin Trans-eliminase.
In the preceding paper (1) a method was described for the purification of the extracellular pectin trans-eliminase of Aspergillus jonsecaeus. The enzyme was purified by a three-step procedure to the point at which other pectic enzymes and cellulase were no longer detectable. The effects of pH, of Na+, Mg++, and Ca++ ions, and of a few anions on the activity of the enzyme were also reported. In ...
متن کاملPurification and Certain Properties of Pectin Trans-eliminase from Aspergillus Fonsecaeus.
In 1960 Albersheim, Neukom, and Deuel (1) reported on an enzyme present in a commercial pectic enzyme preparation (Pectin01 RlO, Rohm and Haas Company, Philadelphia) that degraded the a, 1 + 4-glycokidic bonds in pectin by a trans elimination of the proton on the 5th carbon atom of an anhydromethyl galacturonate unit with the oxygen of the adjacent glycosidic bond. Cleavage of the bonds in pect...
متن کاملPectin methylesterase, metal ions and plant cell-wall extension. Hydrolysis of pectin by plant cell-wall pectin methylesterase.
The hydrolysis of p-nitrophenyl acetate catalysed by pectin methylesterase is competitively inhibited by pectin and does not require metal ions to occur. The results suggest that the activastion by metal ions may be explained by assuming that they interact with the substrate rather than with the enzyme. With pectin used as substrate, metal ions are required in order to allow the hydrolysis to o...
متن کاملAnalysis of the Components Released from Potato Tuber Tissues during Maceration by Pectolytic Enzymes.
Endo-pectin lyase and endo-polygalacturonase of Aspergillus japonicus attack the middle lamella of plant tissue and cause tissue maceration. Galacturonides, neutral sugars, and proteins were released from potato tuber tissues during maceration by both purified enzymes. These three components accounted for 92% of the soluble products. The neutral sugars released were rhamnose, arabinose, and gal...
متن کاملOxidation of paraffins by plant tissues.
Paraffins are fotund in almost every organism, plants (2), animals (13), and microorganismiis (14). The most common biological source of paraffins appears to be the surface lipids of plants (2), the chain length of the most abulndant paraffin being most often C., or C.:. These natural paraffins cant be readily catabolized by soil organisms (5) and to a limited extent bv an,inlals (6). The mecha...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1971
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.35.1157