منابع مشابه
The tryptic digestion of S-aminoethylated ribonuclease.
The peptides produced by the tryptic digestion of S-aminoethylated bovine pancreatic ribonuclease support the generally accepted amino acid sequence for this enzyme and also demonstrate the usefulness as well as some special limitations of the aminoethylation procedure. The relative extents of hydrolysis at different peptide bonds were estimated from the yields of purified peptides. Under the c...
متن کاملThe effect of halogen salts on tryptic digestion.
THE effect of halogen salts on salivary and peptic digestion has been described previously [Clifford, 1925; 1927; 1928], and in this paper an account is given of their action on tryptic digestion. The earliest work on the subject appears to be that of Pfeiffer [1884] who found that, with the exception of sodium carbonate, all salts, especially sodium chloride, retard tryptic digestion. Later, W...
متن کاملTryptic digestion of spectrin in variants of hereditary elliptocytosis.
Spectrin, either in the form of unfractionated low ionic strength extracts of erythrocyte membranes or purified by chromatography on Sepharose (CL)4B, was subjected to tryptic digestion at 0 degrees C. Four patients, each with a different variant of hereditary elliptocytosis, were studied. In one patient, whose erythrocytes showed significant fragmentation on heating on 45 degrees C, such prepa...
متن کاملPhysical properties of small functional units obtained from gastropod mollusc haemocyanins by tryptic digestion.
The haemocyanins of gastropod molluscs are giant proteins of mol.wt. approx. 9000000 whose function is the transport of oxygen in the blood. It is well established that the functional unit, which binds one molecule of 02, is a pair of copper atoms surrounded by a folded polypeptide chain to form a compact domain of mol.wt. approx. 50000 (Van Breemen et al., 1977). However, treatment of the prot...
متن کاملTryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry.
Ubiquitination plays an essential role in maintaining cellular homeostasis by regulating a multitude of essential processes. The ability to identify ubiquitinated proteins by MS currently relies on a strategy in which ubiquitinated peptides are identified by a 114.1 Da diglycine (GG) tag on lysine residues, which is derived from the C-terminus of ubiquitin, following trypsin digestion. In the f...
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ژورنال
عنوان ژورنال: Nature
سال: 1919
ISSN: 0028-0836,1476-4687
DOI: 10.1038/103064d0