M26 recombinational hotspot and physical conversion tract analysis in the ade6 gene of Schizosaccharomyces pombe.
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چکیده
منابع مشابه
M26 recombinational hotspot and physical conversion tract analysis in the ade6 gene of Schizosaccharomyces pombe.
At the ade6 locus of Schizosaccharomyces pombe flanking markers have been introduced as well as five silent restriction site polymorphisms: four in the 5' upstream region and one in the middle of the gene. The mutations ade6-706, ade6-M26 (both at the 5' end) and ade6-51 (middle of the gene) were used as partners for crosses with the 3' mutation ade6-469. From these three types of crosses, wild...
متن کاملActive and inactive transplacement of the M26 recombination hotspot in Schizosaccharomyces pombe.
The ade6-M26 mutation of the fission yeast Schizosaccharomyces pombe creates a meiotic recombination hotspot that elevates ade6 intragenic recombination approximately 10-15-fold. A heptanucleotide sequence including the M26 point mutation is required but not sufficient for hotspot activity. We studied the effects of plasmid and chromosomal context on M26 hotspot activity. The M26 hotspot was in...
متن کاملThe M26 hotspot of Schizosaccharomyces pombe stimulates meiotic ectopic recombination and chromosomal rearrangements.
Homologous recombination is increased during meiosis between DNA sequences at the same chromosomal position (allelic recombination) and at different chromosomal positions (ectopic recombination). Recombination hotspots are important elements in controlling meiotic allelic recombination. We have used artificially dispersed copies of the ade6 gene in Schizosaccharomyces pombe to study hotspot act...
متن کاملGenetic and Physical Analysis of the M 26 Recombination Hotspot of Schizosaccharomyces pombe Alfred
The ade6-M26 mutation of Schizosaccharomyces pombe has previously been reported to stimulate d e 6 intragenic meiotic recombination. We report here that the ade6-M26 mutation is a single G + T nucleotide change, that M26 stimulated recombination within ade6 but not at other distant loci, and that M26 stimulated meiotic but not mitotic recombination. In addition, M26 stimulated recombination wit...
متن کاملHot spots of recombination in fission yeast: inactivation of the M26 hot spot by deletion of the ade6 promoter and the novel hotspot ura4-aim.
The M26 mutation in the ade6 gene of Schizosaccharomyces pombe creates a hot spot of meiotic recombination. A single base substitution, the M26 mutation is situated within the open reading frame, near the 5' end. It has previously been shown that the heptanucleotide sequence 5' ATGACGT 3', which includes the M26 mutation, is required for hot spot activity. The 510-bp ade6-delXB deletion encompa...
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ژورنال
عنوان ژورنال: Genetics
سال: 1994
ISSN: 1943-2631
DOI: 10.1093/genetics/136.1.41