منابع مشابه
Lysozyme-induced polymerization of tubulin. Burial of the colchicine-binding site as a probe.
Ample evidence has accumulated indicating that the information for forming microtubule lattice lies in the tubulin molecule. There are varieties of agents such as Mg2+ [l-3], dimethyl sulfoxide [4], polyethylene glycol, DEAE-dextran [S] and basic proteins [6-91 which could induce microtubule assembly in a purified preparation of tubulin. However, it appears that in those assembly conditions whe...
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Oligoanions such as sodium triphosphate or GTP prevent and/or reverse vinblastine-induced polymerization of tubulin. We now show that the anions of glutamate-rich extreme C termini of tubulin are similarly involved in the regulation of the vinblastine effect. Cleavage of the C termini by limited proteolysis with subtilisin enhances vinblastine-induced tubulin polymerization and abolishes the an...
متن کامل5368 Tubulin Polymerization in Dimethyl Sulfoxide
The self-assembly of tubulin devoid of microtubuleassociated proteins (MAPs) has been studied using a MES buffer containing dimethyl sulfoxide (MezSO). Between 6% and 12% v/v MeZSO, the tubulin forms polymers which resemble microtubules in their morphology and chemical properties. These MezSO microtubules, like normal microtubules, require GTP for assembly and are sensitive to cold, calcium ion...
متن کاملHeterogeneous Tau-Tubulin Complexes Accelerate Microtubule Polymerization.
Tau is an intrinsically disordered protein with a central role in the pathology of a number of neurodegenerative diseases. Tau normally functions to stabilize neuronal microtubules, although the mechanism underlying this function is not well understood. Of note is that the interaction between tau and soluble tubulin, which has implications both in understanding tau function as well as its role ...
متن کاملTubulin polymerization promoting protein 1 (TPPP1)
The eukaryotic cell cycle relies heavily on the mechanical forces vested by the dynamic rearrangement of the microtubule (MT) network. Tubulin Polymerization promoting Protein 1 (TPPP1) alters MT dynamics by driving MT polymerization as well as stabilization, via increasing MT acetylation. It increases MT rigidity, which results in reduced cell proliferation through downregulation of G1/S-phase...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1981
ISSN: 0014-5793
DOI: 10.1016/0014-5793(81)80157-3