Lysophosphatidic Acid Acyltransferase from Meadowfoam Mediates Insertion of Erucic Acid at the sn-2 Position of Triacylglycerol in Transgenic Rapeseed Oil
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چکیده
منابع مشابه
Lysophosphatidic acid acyltransferase from meadowfoam mediates insertion of erucic acid at the sn-2 position of triacylglycerol in transgenic rapeseed oil.
Lysophosphatidic acid acyltransferase acylates the sn-2 hydroxyl group of lysophosphatidic acid to form phosphatidic acid, a precursor to triacylglycerol. A cDNA encoding lysophosphatidic acid acyltransferase was isolated from developing seeds of meadowfoam (Limnanthes alba alba). The cDNA encodes a 281-amino acid protein with a molecular mass of 32 kD. The cDNA was expressed in developing seed...
متن کاملExpression of rapeseed microsomal lysophosphatidic acid acyltransferase isozymes enhances seed oil content in Arabidopsis.
In higher plants, lysophosphatidic acid acyltransferase (LPAAT), located in the cytoplasmic endomembrane compartment, plays an essential role in the synthesis of phosphatidic acid, a key intermediate in the biosynthesis of membrane phospholipids in all tissues and storage lipids in developing seeds. In order to assess the contribution of LPAATs to the synthesis of storage lipids, we have charac...
متن کاملHuman Lysophosphatidic Acid Acyltransferase
Lysophosphatidic acid (1-acyl-sn-glycero-3-phosphate (LPA)) is a phospholipid with diverse biological activities. The mediator serves as an intermediate in membrane phospholipid metabolism but is also produced in acute settings by activated platelets. LPA is converted to phosphatidic acid, itself a lipid mediator, by an LPA acyltransferase (LPAAT). A human expressed sequence tag was identified ...
متن کاملEnvironmental and Induced Mutations Influences on Oil Content, Oleic Acid and Erucic Acid in Rapeseed
A comparative study was conducted to see the mutational analysis for monounsaturated fatty acids in rapeseed (Brassica napus L.) mutant populations. By using gamma irradiation, a range of variability generated for oil content and fatty acid composition in rapeseed seeds. The induced genetic variability was analyzed for two consecutive years in two contrasting environments at elevations of 510 a...
متن کاملBiosynthesis of castor oil: effect of polyamines on the acylation of lysophosphatidic acid at the sn-2 position with ricinoleic acid.
Polyamines with diamine structures of chain length longer than 3C were essential for the synthesis of phosphatidic acid (PA) from ricinoleoyl-CoA and lysophosphatidic acid (LPA) by the castor LPA acyltransferase reaction, suggesting that polyamines modulate enzyme affinity for the acyl-CoA substrate in vivo.
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1995
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.109.4.1389