Lymphoid Follicle Transcriptome Reveals Maladaptive Immune Responses In Severe Chronic Obstructive Pulmonary Disease (COPD).
نویسندگان
چکیده
Abstract Rationale: Increases in the number and size of lymphoid follicles (LFs) are present severe stages COPD have not been fully characterized. HLA complex is crucial enabling immune system to recognize “self” versus “non-self” antigens plays a major role responses cigarette smoke exposure. Methods: Lung from patients with milder (GOLD I–II) more III–IV) were sampled using GeoMX® Digital Spatial Transcriptional Profiling. Data analyzed normalized GeoMx® DSP Analysis Suite. Results: Pathway analysis showed that B cell activation pathways had highest fold enrichment. GOLD I–II compared III–IV expression HLA-Cand its ligands (KIR2DS2, KIR2DS1, KIR3DL1; p<0.0001) which facilitate CD8-mediated cytotoxicity, HLA-Gand receptor LILRB4(p=0.01) key modulators tolerance. enrichment LFs, upregulation transcription factors (HIF1A, IRF1, NFYBand NFYC) assembly components (CALR, TAPBP, ERP27, MARCHF1, B2M, CIITA) (p<0.01). Compared I–II, LFs much higher HLAs involved autoimmune self-antigens adaptive evasion, such HLA-DRA, HLA-DRB1, HLA-DPA1, HLA-DPB1, HLA-DQA1, HLA-DQB1, HLA-Eand HLA-B(p<0.0001). Conclusions: We show for first time characterized by associated evasion responses, whereas induction controlled tolerance mechanisms R01 HL 149744-04/06
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ژورنال
عنوان ژورنال: Journal of Immunology
سال: 2023
ISSN: ['1550-6606', '0022-1767']
DOI: https://doi.org/10.4049/jimmunol.210.supp.149.15