Lymphocyte transformation test: History and current approaches

Drug-induced hypersensitivity reactions encompass a variety of different clinical phenotypes ranging from harmless rashes to fatal reactions. They can be classified into allergic (i.e. drug allergy) and non-allergic hypersensitivity). Drug allergies in turn either antibody (e.g. IgE) or T cell-mediated. One the diagnostic tools for vitro detection allergy is lymphocyte transformation test (LTT) which based on activation expansion drug-specific memory cells following co-incubation patient's peripheral mononuclear (PMBC) with suspected vitro. The read-out parameter classical LTT cell proliferation measured as counts per minute addition radiolabeled thymidine culture. However, course time modifications regard parameters methods have been proposed. Likewise, variations platform itself described literature. This review article describes development its use context summarizes published over time.