Lower temperature influences Cauliflower mosaic virus systemic infection

Cauliflower mosaic virus isolate CMV-1.

Nuclei purified from cauliflower mosaic virus-infected turnip leaves contain subgenomic, covalently closed circular cauliflower mosaic virus DNAs.

Nuclei isolated from cauliflower mosaic virus (CaMV) infected turnip leaves contain subgenomic CaMV DNA species in addition to the genome length CaMV DNA. These subgenomic CaMV DNA species are present as covalently closed circles (form I), relaxed circles (form II) and linear (form III) molecules. The subgenomic form I DNA species range in size from about 10% of genome length to nearly genome l...

A second cauliflower mosaic virus gene product influences the structure of the viral inclusion body.

We have used electron microscopy of thin sections and experiments on isolated viroplasms to compare the properties of four strains of cauliflower mosaic virus (CaMV), three of which were partially or completely deleted in open reading frame (ORF) II. Our results confirm that this gene is required for aphid transmissibility and show that the product of ORF II influences the firmness with which v...

Biochemical characterization of the helper component of Cauliflower mosaic virus.

The helper component of Cauliflower mosaic virus is encoded by viral gene II. This protein (P2) is dispensable for virus replication but required for aphid transmission. The purification of P2 has never been reported, and hence its biochemical properties are largely unknown. We produced the P2 protein via a recombinant baculovirus with a His tag fused at the N terminus. The fusion protein was p...

Infection of Protoplasts from Several Brassica Species with Cauliflower Mosaic Virus Following Inoculation Using Polyethylene Glycol

Using a polyethylene glycol-mediated uptake procedure, protoplasts from a range of Cruciferous species were infected with cauliflower mosaic virus; they include Moricandia arvensis, a plant not k n o w n to be susceptible. With this technique > 90~o of turnip protoplasts were infected, each producing an average of 104 to 105 viral genome equivalents. Filter hybridization and fluorescent antibod...