Low-copy nuclear primers and ycf1 primers in Cactaceae
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چکیده
منابع مشابه
Primers for low-copy nuclear genes in the Melastomataceae1
PREMISE OF THE STUDY Low-copy nuclear gene primers were developed for phylogenetic studies across the Melastomataceae. METHODS AND RESULTS Total genomic libraries from eight species in the Melastomataceae along with one transcriptome were used for marker identification and primer design. Eight exon-primed intron-crossing markers were amplified with success in taxa of nine tribes in the Melast...
متن کاملPrimers for low-copy nuclear genes in Metrosideros and cross-amplification in Myrtaceae1
UNLABELLED PREMISE OF THE STUDY Primers were developed to amplify low-copy nuclear genes in Hawaiian Metrosideros (Myrtaceae). • METHODS AND RESULTS Data from a pooled 454 Titanium run of the partial transcriptomes of four Metrosideros taxa were used to identify the loci of interest. Ten exon-primed intron-crossing (EPIC) markers were amplified and sequenced directly with success in Metros...
متن کاملPrimers for low-copy nuclear genes in the Hawaiian endemic Clermontia (Campanulaceae) and cross-amplification in Lobelioideae1
UNLABELLED PREMISE OF THE STUDY Primers were developed to amplify 12 intron-less, low-copy nuclear genes in the Hawaiian genus Clermontia (Campanulaceae), a suspected tetraploid. • METHODS AND RESULTS Data from a pooled 454 titanium run of the partial transcriptomes of seven Clermontia species were used to identify the loci of interest. Most loci were amplified and sequenced directly with ...
متن کاملPrimers for Castilleja and their utility across Orobanchaceae: II. Single-copy nuclear loci1
PREMISE OF THE STUDY We developed primers targeting nuclear loci in Castilleja with the goal of reconstructing the evolutionary history of this challenging clade. These primers were tested across other major clades in Orobanchaceae to assess their broader utility. METHODS AND RESULTS We assembled low-coverage genomes for three taxa in Castilleja and developed primer combinations for the singl...
متن کاملTwenty-seven low-copy nuclear primers for Lindera obtusiloba (Lauraceae): A Tertiary relict species in East Asia1
Premise of the study To investigate a more detailed evolutionary history of Lindera obtusiloba (Lauraceae) and other Lindera species, polymorphic low-copy nuclear primers were developed. Methods and Results Unigenes of the L. obtusiloba transcriptome greater than 800 bp in length were randomly chosen for initial design of 168 primers. Agarose gel electrophoresis and Sanger sequencing were use...
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ژورنال
عنوان ژورنال: American Journal of Botany
سال: 2012
ISSN: 0002-9122
DOI: 10.3732/ajb.1200128