Low blood levels of the lncRNA MRPL20-AS1 are associated with severe obstructive sleep apnea

Abstract Introduction Obstructive sleep apnea syndrome (OSAS) is the most common disorder and it associated with arterial hypertension, heart failure, coronary artery disease as well atrial fibrillation. The underlying pathomechanisms for this association are only incompletely understood. In recent years long non-coding RNAs (lncRNA) have been shown to be involved in various cardiovascular pathologies. aim of study identify lncRNAs which OSAS order provide potential therapeutic targets. Methods results tackle issue, we included 23 Patients a suspected pilot sampled blood on evening before morning after polysomnographic analysis. Citrate was used inhibit clotting cellular components were removed by centrifugation, plasma stored at −80°C. One patient had no sign indicated an Apnea/Hypopnea-Index (AHI) <5 per hour therefore excluded. remaining 22 patients mean age 44.45 AHI 28.38 hour. Three female nineteen male. four those high levels, performed RNA sequencing analysis from citrate found MRPL20-AS1 significantly regulated lncRNA night (A). Low coding confirmed silico via Coding-Potential Assessment Tool (http://lilab.research.bcm.edu/) resulting probability 0.06148. Then levels measured qPCR patients. From 19 patients, obtained evaluable results. We that tendency lower assessment (B). Interestingly inversely correlated (C). This indicates severe low our cohort (D). further investigate these vitro, subjected human endothelial cells (HCAECs) hypoxia (1% 5% O2) 24 h. leads significant downregulation HCAECs (E). Conclusion nocturnal hypoxia. downregulated can useful suffering OSAS. Further investigations needed elucidate biological function context target counteract effects Funding Acknowledgement Type funding sources: Public Institution(s). Main source(s): Unviversity BonnUniversity Cologne