Locations of bacteriophage T4 origins of replication
نویسندگان
چکیده
منابع مشابه
Identification of the origins of T4 DNA replication.
Two physical origins of T4 DNA replication were determined by hybridization of viral DNA prepared 2.5 min after infection to a display of total T4 DNA. This is the earliest time after T4 infection of Escherichia coli at 37 degrees C that labeled and hybridizable DNA can be detected. The two origins, separated by about 25 kilobases, were identified and localized in the early region of the T4 map...
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Bacteriophage T4 UvsW protein is involved in phage recombination, repair and the regulation of replication origins. Here, we provide evidence that UvsW functions as a helicase. First, expression of UvsW allows growth of an (otherwise inviable) Escherichia coli recG rnhA double mutant, consistent with UvsW being a functional analog of the RecG helicase. Second, UvsW contains helicase sequence mo...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1985
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.54.2.271-277.1985