Localization of the type I ribosome-inactivating protein, luffin, in adult and embryonic tissues of Luffa cylindrica L. Roem.
نویسندگان
چکیده
منابع مشابه
Complete amino acid sequence of luffin-b, a ribosome-inactivating protein from sponge gourd (Luffa cylindrica) seeds.
The complete amino acid sequence of luffin-b has been determined. All the twenty-seven tryptic peptides were isolated by reverse-phase HPLC from the tryptic digests of intact luffin-b and one of its CNBr fragments (CB4), and sequenced using the DABITC/PITC double coupling method. The overlap of these peptides was achieved by analyzing the CNBr fragments and their chymotryptic peptides. Luffin-b...
متن کاملthe investigation of the relationship between type a and type b personalities and quality of translation
چکیده ندارد.
Hepatoprotective activity of Luffa cylindrica (L.) M. J. Roem. leaf extract in paracetamol intoxicated rats
Methanolic extract of Luffa cylindrica (L.) M. J. Roem. leaves (MELC) was evaluated for its hepatoprotective potential against paracetamol intoxicated wistar rats. Administration of MELC and standard drug silymarin showed significant (P <0.05) hepatoprotective protection in experimental animals. The study was conducted by estimating serum enzyme activities of serum glutamate oxaloacatate transa...
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هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
Optimization of EnBase Fed-Batch Cultivation to Improve Soluble Fraction Ratio of α-Luffin Ribosome Inactivating Protein
Background: The increase of the protein expression via ribosomal manipulation is one of the suggested cellular mechanisms involved in EnBase fed-batch mode of cultivation. However, this system has not been implemented for cytotoxic proteins.Objectives: Here, the expression pattern of α-Luffin, a ribosome inactivation protein (RIP) with an innate toxicity,...
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ژورنال
عنوان ژورنال: Journal of Experimental Botany
سال: 1999
ISSN: 0022-0957,1460-2431
DOI: 10.1093/jxb/50.334.573