Localization of Core Spindle Pole Body (SPB) Components during SPB Duplication in Saccharomyces cerevisiae
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Localization of Core Spindle Pole Body (SPB) Components during SPB Duplication in Saccharomyces cerevisiae
We have examined the process of spindle pole body (SPB) duplication in Saccharomyces cerevisiae by electron microscopy and found several stages. These include the assembly, probably from the satellite, of a large plaque-like structure, the duplication plaque, on the cytoplasmic face of the half-bridge and its insertion into the nuclear envelope. We analyzed the role of the main SPB components i...
متن کاملSpc42p: A Phosphorylated Component of the S. cerevisiae Spindle Pole Body (SPB) With an Essential Function During SPB Duplication
The 42-kD component of the S. cerevisiae spindle pole body (SPB) localizes to the electron-dense central plaque of the SPB (Rout, M.P., and J.V. Kilmartin. 1991. Cold Spring Harbor Symp. Quant. Biol. 56:687--691). We have cloned the corresponding gene SPC42 (spindle pole component) and show that it is essential. Seven temperature-sensitive (ts) mutants in SPC42 were prepared by error-prone PCR....
متن کاملSpc42p: a phosphorylated component of the S. cerevisiae spindle pole body (SPD) with an essential function during SPB duplication
The 42-kD component of the S. cerevisiae spindle pole body (SPB) localizes to the electron-dense central plaque of the SPB. We have cloned the corresponding gene SPC42 (spindle pole component) and show that it is essential. Seven temperature-sensitive (ts) mutants in SPC42 were prepared by error-prone PCR. We found that a change to a proline residue in a potential coiled-coil region of Spc42p w...
متن کاملChanges in the nuclear envelope environment affect spindle pole body duplication in Saccharomyces cerevisiae.
The Saccharomyces cerevisiae nuclear membrane is part of a complex nuclear envelope environment also containing chromatin, integral and peripheral membrane proteins, and large structures such as nuclear pore complexes (NPCs) and the spindle pole body. To study how properties of the nuclear membrane affect nuclear envelope processes, we altered the nuclear membrane by deleting the SPO7 gene. We ...
متن کاملThe Saccharomyces cerevisiae spindle pole body duplication gene MPS1 is part of a mitotic checkpoint
M-phase checkpoints inhibit cell division when mitotic spindle function is perturbed. Here we show that the Saccharomyces cerevisiae MPS1 gene product, an essential protein kinase required for spindle pole body (SPB) duplication (Winey et al., 1991; Lauze et al., 1995), is also required for M-phase check-point function. In cdc31-2 and mps2-1 mutants, conditional failure of SPB duplication resul...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1999
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.145.4.809