LncRNA SNHG1 upregulates FANCD2 and G6PD to suppress ferroptosis by sponging miR-199a-5p/3p in hepatocellular carcinoma

Ferroptosis is a form of regulated cell death (RCD) triggered by iron-dependent lipid peroxidation and closely associated with the occurrence progression hepatocellular carcinoma (HCC). The lncRNA SNHG1 (small nucleolar RNA host gene 1) has been shown to play an oncogenic role in HCC, but its function RCD other than autophagy apoptosis still unknown. Here, we investigated correlation between 156 typical markers five types based on sequencing data from Cancer Genome Atlas database showed negative regulators ferroptosis FANCD2 (Fanconi anemia complementation group D2) G6PD (glucose-6-phosphate dehydrogenase) be most highly fifth correlating factors SNHG1, respectively. A competitive endogenous network – miR-199a-5p/3p FANCD2/G6PD was constructed bioinformatically. In vitro experiments that overexpression miR-199a precursor led decrease expression FANCD2, G6PD, whereas knockdown decreased increased levels miR-199a-5p miR-199a-3p HCC cells (Huh7 HepG2). addition, erastin-mediated ferroptosis, iron accumulation, peroxidation. These results suggest upregulates sponging miR-199a, thereby inhibiting HCC. Moreover, signature identified as being overall survival immunological microenvironment Collectively, this study SNHG1–miR-199a–FANCD2/G6PD axis which potential marker for prognosis therapy tumor.