LncRNA HOXA-AS3 promotes gastric cancer progression by regulating miR-29a-3p/LT?R and activating NF-?B signaling

Abstract Background Gastric cancer (GC) is among the most common and deadliest cancers globally. Many long non-coding RNAs (lncRNAs) are key regulators of GC pathogenesis. This study aimed to define role HOXA-AS3 in this oncogenic context. Methods Levels expression were quantified via qPCR. The effects knockdown on cells function evaluated vitro using colony formation assays, wound healing assays transwell assays. Subcutaneous xenograft tail vein injection tumor model systems generated nude mice assess lncRNA vivo. localization within was confirmed by subcellular fractionation, predicted microRNA (miRNA) targets its ability modulate downstream NF-?B signaling luciferase-reporter immunofluorescent staining, western blotting. Results tissues exhibited significant upregulation ( P < 0.05), levels found be correlated with size, lymph node status, invasion depth, Helicobacter pylori infection status. Knocking down disrupted cell proliferation, migration, metastasis vivo . At a mechanistic level, we that able sequester miR-29a-3p, thereby regulating LT?R modulating GC. Conclusion HOXA-AS3/miR-29a-3p/LT?R/NF-?B regulatory axis contributes progression GC, offering novel target for prognosis treatment