lncRNA APOC1P1-3 promoting anoikis-resistance of breast cancer cells

Abstract Background Anoikis resistance plays a critical role in the tumor metastasis by allowing survival of cancer cells systemic circulation. We previously showed that long non-coding RNAs APOC1P1-3 (lncRNA APOC1P1-3) inhibit apoptosis breast cells. In this study, we explored its anoikis resistance. Methods induced two cell lines (MCF-7 and MDA-MB-231) under anchorage-independent culture conditions studied lncRNA effects on apoptosis. Using Dual-Luciferase activity assay, determined whether it specifically binds to miRNA-188-3P. further lung injecting MDA-MB-231 MDA-MB-231-APOC1P1-3-knock-down female BALB/c nude mice. Results found suppressed early these (demonstrated gain or loss their function, respectively) promoted via reducing activated- Caspase 3, 8, 9 PARP. Moreover, target miRNA-188-3p acting as “sponge” block inhibition Bcl-2 (an anti-apoptosis protein). Conclusions Our study supports theory can promote development binding Bcl-2.